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dc.contributor.authorWanga, B. N.
dc.contributor.authorAkenga, T.
dc.contributor.authorImbuga, M.
dc.contributor.authorGitonga, L.
dc.contributor.authorOlubayo, F.
dc.contributor.authorNamungu, P.
dc.date.accessioned2023-01-12T08:10:14Z
dc.date.available2023-01-12T08:10:14Z
dc.date.issued2006
dc.identifier.citationJournal of Agriculture, Science and Technology Vol. 8 (1) 2006: pp. 1-14en_US
dc.identifier.issn1561-7645
dc.identifier.urihttps://www.ajol.info/index.php/jagst/about
dc.identifier.urihttps://karuspace.karu.ac.ke/handle/20.500.12092/2759
dc.descriptionAntimicrobial acitivity of extractsen_US
dc.description.abstractThe dichloromethane extracts of the root and leaf of Tephrosia vogelii Hook F. (Family: Papilionaceae) grown in Kenya, were subjected to antimicrobial assays against Staphylococcus aureus, Escherichia coli and Fusarium phoseolida. The minimum inhibition concentrations (MIC) ranged between 0.25 to 6.4 μg/ml for all the microorganisms tested. The crude root and leaf extracts were also toxic to the brine shrimps, organisms used as indicators of toxicity (LC50: 0.960; 0.958μg/ml,respectively). Chromatographic separation led to the identification of (6aß, 12aß, 5'ß)-6a, 12a, 4',5'-tetra hydro-2,3-dimethoxy 5'-ß- isopropenyl–furano (3',2': 8,9)-6H-rotoxen-12-one, (rotenone) in 9.75% yield, (roots), and 11.75% (leaves). Characterisation was achieved by use of spectroscopic techniques and comparison with rotenone standard and literature data. Novel HPLC conditions for purification of rotenoids were established. The MIC of rotenone against the tested microbes was found to be 5.2 and 1.0 μg/ml for Staphylococcus aureus and Escherichia coli respectively, while the LC50 against the brine shrimps was found to be 3.20 μg/ml. The findings indicate that the extracts of T. vogelii contain metabolites that have antimicrobial activity comparable to other medicinal plants.en_US
dc.language.isoenen_US
dc.publisherJomo Kenyatta University of Agriculture & Technology (JKUAT), Kenyaen_US
dc.subjectTephrosia vogeliien_US
dc.subjectRotenoneen_US
dc.subjectStaphylococcus aureusen_US
dc.subjectEscherichiacolien_US
dc.subjectFusarium phoseolidaen_US
dc.titleAntimicrobial acitivity of extracts from Tephrosiavogelii Hook F.en_US
dc.typeArticleen_US


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