Department of Agricultural Sciences

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Author: search.filters.author.Kiplagat, OliverHas files: Yes

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    Evaluation of drought tolerance in mutant Kenyan bread wheat (Triticum aestivum L.) using in vitro techniques
    (INNSPUB, 2016-08-31) Githinji, Gerald Gikonyo; Kinyua, Miriam; Kiplagat, Oliver; Birithia, Rael
    Wheat (Triticum aestivum L) is widely cultivated as a small-grain cereal. In Kenya, it is ranked second after maize in its contribution towards food security. Biotic stress conditions such as drought cause extensive losses to agricultural production worldwide. In Kenya, arid and semiarid lands represent 83% of total land area, which experience frequent crop failure due to drought stress. Developing drought-tolerant wheat genotypes has been the focus of many wheat improvement programs. Few drought tolerant varieties are available for commercial production in Kenya. Hence, there is need to develop more drought tolerant wheat varieties. The objective of this study was to screen for drought resistance in two mutant wheat lines in vitro using Polyethylene Glycol (PEG). Four wheat germplasm were tested for drought tolerance using -3.0, -9.0 and -15.0 PEG-6000 concentrations and the data was recorded on various seedling parameters including root length, shoot length and root length /shoot length ratio. The experiment was carried out in three replicates using completely randomized design. Data was subjected to analysis of variance (ANOVA) using GENSTAT 12th edition. Correlation was done by Pearson Correlation Coefficients to determine significant associations among the different variables. Results indicated that there was a significant difference (p=0.05) between Mutant 1 and Mutant 2 having longer roots, shoots and a root to shoot ratio compared to Chozi and Duma in the different PEG concentrations used. Hence, the two mutant lines are possible candidates for varieties that can be grown in ASALs regions in Kenya.
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    Evaluation of drought tolerance in some Kenyan bread wheat (Triticum Aestivum L.) at seedling stage
    (2019-11-02) Githinji, Gerald G.; Kinyua, Miriam; Kiplagat, Oliver; Birithia, Rael K.
    Wheat (Triticum aestivum L) is the second largest contributor to food security in Kenya. However, water deficiency retards plant growth and productivity significantly. With few drought tolerant varieties available, there is need to develop more drought tolerant wheat varieties. Objective of this study was to screen for drought tolerance in the mutant wheat lines at seedling stage. Seeds of two wheat varieties were subjected to gamma radiation at an irradiation dose of 300 gy (gray). The mutants and two local varieties – Chozi and Duma (controls) were sown in polythene bags in a complete randomized design and screened for various seedling traits. Data were analysed using ANOVA and Pearson correlation. Results indicated that Mutant 1 and Mutant 2 had high emergence percentage, emergence index, energy emergence and per cent seedling recovery compared to local varieties Chozi and Duma. Emergency percentage showed a positive correlation with the following parameters; emergence index (r = 0.965); Energy of emergency (r = 0.990) and Percentage seedling recovery (r = 0.941). It was recommended that the mutant wheat be screened for other biotic and abiotic stresses that affect wheat production in Kenya.