Department of Physical Sciences

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Author: search.filters.author.Kavoo, Agnes Mumo

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    A Gold Nanoparticle-Based Colorimetric Probe for Detection of Gibberellic Acid Exuded by Ralstonia solanacearum Pathogen in Tomato (Solanum lycopersicum L.)
    (International Journal of Horticultural Science and Technology, 2021-01-27) Aoko, Ivy Lynn; Ondigo, Dezzline; Kavoo, Agnes Mumo; Wainaina, Cornelius; Kiirika, Leonard
    We report a simple colorimetric probe based on gold nanoparticles (AuNPs) for detecting Ralstonia solanacearum. The AuNPs were synthesized through reduction with citrate ion and characterized by ultraviolet-visible spectroscopy (UV-vis), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The freshly synthesized AuNPs were brick red due to an intense surface plasmon absorption band at 520 nm. Upon interaction with synthetic gibberellic acid (GA3), a bathochromic shift occurred in the surface plasmon resonance (SPR) peak of AuNPs to higher wavelengths. The 'eye-ball' limit of detection was 0.2 ppm. This shift was accompanied by a change in the color of the AuNPs from brick red to purple. Soil samples were collected from the rhizosphere of tomato plants, exhibiting bacterial wilt symptoms and pure cultures of Ralstonia solanacearum isolated using a modified Kelman’s TZC medium. Gibberellins (GA) were extracted from the culture of R. solanacearum using ethyl acetate and characterized using fourier transform infrared spectroscopy (FT-IR). AuNP solution aggregation was induced by GA-mediated R. solanacearum. A color change from brick red to purple was also observed. The results illustrated the use of both SPR wavelength-shift sensing and visual color change to detect molecules of biological relevance
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    Water Resources and Management under Increasing Urban Demography: A Kenyan Perspective—A Review
    (Journal of Water Resource and Protection, 2018) Ondigo, Deziline Adhiambo; Kebwaro, Jeremiah; Kavoo, Agnes Mumo
    The United Nations classifies Kenya as a water-scarce country since it has less than 1000 m3 per capita of renewable freshwater supplies. Numerous factors including global warming, contamination of drinking water, and a lack of investment in water resources have aggravated the water crisis in Kenya. Estimates indicate that only about 56% of its population has access to safe water supply. Like many developing countries, Kenya recognizes the crucial role of water in realizing its development goals. Its economic performance and poverty reduction are critically dependent on clean water availability for agriculture, industrialization, energy production and tourism among others. Similar to most developing countries, Kenya suffers from lack of human, monetary and institutional capabilities to efficiently provide clean and sufficient water to its citizens. The water shortage in the major cities is acute and chronic and has continued to worsen with increasing urbanization, water pollution and encroachment of water catchment areas by humans and invasive plant species. Despite the water challenges facing the urban populations, Kenya possesses sufficient water resources to meet demand if the available resources are properly managed. Several initiatives are being put in place in Kenya to mitigate the water challenges and protect water resources in Kenya. These include enacting of water policies to protect water catchment areas, reduce pollution and enhance access to clean water.
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