School of Agriculture and Biotechnology
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Item Evaluation of Ephestia kuehniella and Corcyra cephalonica as hosts for mass rearing trichogramma species nr. Mwanzai and trichogrammatoidea species nr. Lutea(2008-01-16) Migiro, L. N.; Gitonga, L. M.; Sithanantham, S.Production of vegetables free of chemical pesticides requires, among other things, use of biocontrol agents that can be effectively and efficiently produced in large quantities. Two factitious hosts were evaluated for their suitability in mass rearing of two native Trichogrammatids. Egg cards bearing host eggs were separately offered to female parasitoids in glass vials and allowed to parasitise for 24 hours. New egg cards were offered daily till death of the female parasitoid. The number of eggs parasitised, emerged adult progeny and female progeny differed significantly between the two parasitoids, with Trichogramma sp. nr. Mwanzai appearing to be superior to Trichogrammatoidea sp. nr. lutea.There were no differences in the mass production efficiency and quality of progeny between the Ephestia kuehniella and Corcyra cephalonica. The two hosts can be effectively used for mass production of either of the parasitoids.Item Biological control agents of aphids (Homoptera: Aphididae) on potatoes (Solanum tuberosumL) in Kenya(2010) Machangi, J. M.; Gitonga, L. M.; Nderitu, J. H.; Maniania, N. K.; Kabira, J. N.A field survey was conducted in four major potato growing areas in Kenya (Kiambu, Nyandarua, Meru and Molo Districts) during January and February, 2008 (short rains crop) and June 2008 (long rains crop) to determine the occurrence of predators, parasitoids and pathogens of the aphids Myzus persicae Sulzer and Aphis gossypii Glover in potato crops. In each of the four areas, 30 potato farms distributed in different parts of the survey area were selected at random for the surveys. Insects collected were brought to the laboratories at the International Centre of insect physiology and Ecology (icipe), Nairobi, for identification and fungal infection. For isolation of fungal pathogens, dead aphids were transferred on moist filter paper placed on sterile Petri dishes to allow the growth of the fungus on the surface of the cadaver, after which fungus was transferred on artificial media for isolation. Four aphid species, M. persicae, A. gossypii, Macrosiphum euphorbiae Thomas and Aulacorthum solani Kaltenbach were identified in all the four survey areas. Sixteen predator species of the aphids were identified. The most prevalent were the ladybeetles Harmonia axyridis (Pallas) and Hippodamnia convergens Guérin-Méneville (Coleoptera: Coccinellidae), followed by the minute pirate bugs, Orius spp. (Heteroptera: Anthocoridae) and the aphid eating gall midge Aphidoletes aphidimyza Rondani (Diptera: Cecidomyiidae). Three hymenopteran parasitoid species (Braconids followed by Chalcids then the Ichneumonids) and four fungal pathogen species, Beauveria bassiana Balsamo (Ascomycota: Hypocreales), followed by Verticillium lecanii Zimmermann (Hypocreales: Incertae sedis), Metarhizium anisopliae (Metsch.) Sorokin (Deuteromycotina: Hyphomycetes) then Pandora neoaphidis (Remaudière &Hennebert) Humber (Zygomycetes:Entomophthorales) were identified in all four areas of the field survey.Item Evaluation of feed for rearing corcyracephalonica and optimisation of host and parasitoid ages for mass-production of trichogramatid parasitoids(2008) Migiro, L. N.; Gitonga, L. M.; Sithanantham, S.Item The Critical Onion Growth Stages For Selective Protection From Thrips Tabaci Lindeman (Thysanoptera:Thripidae) Infestation(Kenya Agricultural Research Institute, Nairobi (Kenya), 2006-01) Waiganjo, M. M.; Sithanantham, S.; Mueke, J. M.; Gitonga, L. M.Field trials were conducted during 2000-2001 at KARIandrsquo;s Mwea-Tebere and Kiboko farms to establish the effect of onion thrips, Thrips tabaci Lindeman on onion bulb yields and to identify the critical onion growth stage for selective protection from the pest infestation. Yield reductions of 59 and 27% were recorded in the first and second crops respectively at Mwea-Tebere, but no significant yield reductions were observed in a third crop grown at Kiboko with low thrips infestation. A significant onion yield response to thrips infestation was observed during the 2nd month after transplanting (bulb-formation) and 3rd month after transplanting (bulb-enlargement), but no significant response was observed during the 1st month (pre-bulbing) and 4th month (maturation stage). Onion protection during bulb-formation and enlargement stages only, resulted in higher marginal returns than full season pesticide application. The studies suggest that control recommendations for onion could be refined to optimize need-based protection.Item Effect of temperature on the development of Orius albidipennis Reuter, a predator of the African legume flower thrips, Megalurothrips sjostedti Trybom(2002) Gitonga, L. M.; Löhr, B.; Overholt, W. A.; Magambo, J. K.; Mueke, J. M.The effects of temperature on the development of Orius albidipennis (Reuter) (Hemiptera: Anthocoridae), reared on its prey, Megalurothrips sjostedti (Trybom) (Thysanoptera: Thripidae), were studied in the laboratory. Nymphal mortality was 87, 48 and 38 % at 20, 25 and 30 °C, respectively. With the exception of first-instar nymphs, percentage mortality was lowest at 25 °C. Pre-oviposition period and longevity decreased with an increase in temperature. Mean daily and total fecundity were 2.1 and 76.4 respectively at 25 °C. The mean developmental period from oviposition to adult eclosion was 27.7, 14.1 and 10.9 days at 20, 25 and 30 °C respectively. There was a linear relationship between temperature and developmental rate (1 /day) of O. albidipennis. Lower thermal thresholds were 13.8, 13.5, 12.7, 15.0, 13.8 and 12.5 °C for eggs and nymphal stages 1 to 5, respectively, with the corresponding average degree-day requirement of 46.9, 42.6, 33.3, 29.2, 26.1 and 51.4 days, respectively. The implications of these results for biological control of thrips are discussed.