School of Agriculture and Biotechnology

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    Home Sustainable Management of Invasive Pests in Africa Chapter Influence of Predatory Mites, Phytoseiulus longipes Evans. on the Within-Plant Diurnal Migration and Distribution of the Red Spider Mite, Tetranychus evansi, Baker and Pritchard on African Nightshade, Solanum scabrum
    (Springer, 2020-07-14) Kungu, Miriam; Subramanian, Sevgan; Salifu, Daisy; Fiaboe, Komi K. M.; Azandémè-Hounmalon, Ginette Y.; Gitonga, Linus; Onyambu, Gladys K.; Deletre, Emilie; Martin, Thibaud
    Understanding the within-plant diurnal migration and distribution of the Red spider mite, Tetranychus evansi Baker and Pritchard, in the presence of the predatory mite Phytoseiulus longipes Evans, on African nightshade, S. scabrum, is critical in developing an Integrated Pest Management (IPM) strategy for the pest. The T. evansi density, day and night cycles, and presence of P. longipes, on the within-plant migration and distribution of T. evansi on African nightshade were hence investigated. The results indicated that T. evansi does not exhibit a circadian migratory movement pattern on S. scabrum at lower densities (50 and 100 mites). However, T. evansi was observed to have a density-dependent collective displacement and distribution to the top of the plant, as densities increased (300 and 600 mites). The presence of P. longipes on the plant enhanced the within-plant migration of T. evansi, even at low densities (50 and 100 mites). However, there was no apparent pattern of migration and movement that was observed within the plant. The increased within-plant movement of T. evansi in presence of the predatory mites and collective displacement of T. evansi at higher densities indicate a potential for developing an IPM strategy using the acaricide treated net, which is discussed further.
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    Evaluation of biological attributes of a native egg parasitoid, Trichogrammatoidea sp. nr. lutea (girault), kenyan accessions
    (2012-09-10) Lagat, Zipporah Osiemo; Gitonga, Linus; Muya, Shadrack Muvui; Ng ' Endo, Rossa
    Objective: This study was carried out to evaluate the biological attributes that is, parasitism, adult progeny production, and sex ratio of 21 strains of a native egg parasitoid Trichogrammatoidea sp. nr. lutea Girault (Hymenoptera: Trichogrammatidae) collected from Plutella xylostella Linnaus (Lepidoptera: Yponomeutidae) in coastal Kenya, reared on Corcyra cephalonica Sainton (Lepidoptera: Pyralidae) in the laboratory. Methods:Atotal of 21 accessions of T.sp. nr. lutea were assembled from the Kenyan coast (Shimba hills and Muhaka sites). Preliminary experiments to select the best performers among the 21 strains, based on biological attributes such as adult longevity, parasitism, emergence, adult progeny produced and progeny sex ratio were conducted under laboratory conditions (temperature of 27±2°C and 65±10% RH). Results: There were significant differences (P<0.05) in all biological attributes studied among the strains. The overall mean adult longevity was 9.31 days. Parasitism, emergence and sex ratio per adult female were 20.2%, 75.56% and 37.77% respectively. The wasp age influenced all the attributes tested significantly (P<0.05) of T.sp. nr. lutea. Conclusion: Four accessions (SH-1 and 4, MK-1 and 3) were selected and recommended from this experiment based on adult longevity, parasitism, adult progeny produced and sex ratio for further evaluation. The results from this study will be useful for mass rearing purposes as well as for future field release programmes
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    Preventive control alternatives to routine foliar spray against Thrips tabaci in onions
    (2008-10) Waiganjo, M.; Sithanantham, S.; Gitonga, Linus; Mueke, J. M.
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    Scope for genetic enhancement of the parasitisation potential of four native strains of Trichogrammatoidea sp. nr. lutea Girault (Hymenoptera: Trichogrammatidae
    (Taylor & Francis, 2007-09) Baya, Joseph M.; Sithanantham, S.; Gitonga, Linus; Osir, Ellie
    In response to emerging interest in commercial mass production of Trichogramma for Helicoverpa armigera biocontrol in eastern Africa, laboratory experiments were undertaken to assess the scope for genetic enhancement of the parasitisation potential of native strains of the local common trichogrammatid species, Trichogrammatoidea sp. nr. lutea. Four promising strains (ex-Kilifi – Kilifi District, ex-Kwa Chai – Kibwezi District, ex-Rarieda – Bondo District and ex-Ebuhayi, Kakamega District) were tested for cross-mating in reciprocal combinations with focus on fecundity and progeny female ratio. While all the crosses resulted in F1 progeny of both sexes, significant differences were observed between homogamic and reciprocal heterogamic crosses in fecundity, progeny production, proportion of female progeny and adult longevity. Among all the crosses, the cross between ex-Rarieda strain females and ex-Kilifi strain males resulted in progeny that was significantly superior in fecundity and progeny female ratio. Conversely, Kilifi strain females crossed to males from ex-Rarieda strain gave rise to progeny with relatively low fecundity and female ratio. There were significant differences between homogamic crosses and most reciprocal heterogamic crosses in the major biological attributes. Genotypic and phenotypic variance-covariance matrices generated for six life-history traits showed high positive correlations for most traits in both inbred (P<0.05) and reciprocal heterogamic crosses (P<0.05 and P<0.001). Fecundity and number of female offspring were the most important factors in the heterogamic crosses. The results confirmed the scope for genetic enhancement through inter-strain crossing for improving the field impact potential of T. sp. nr. lutea being targeted for commercial mass production.
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    SUSCEPTIBLE ONION GROWTH STAGES FOR SELECTIVE AND ECONOMIC PROTECTION FROM ONION THRIPS INFESTATION
    (Karatina University, 2008-03) Waiganjo, M.M; Mueke, J.M.; Gitonga, Linus
    The growth of the bulb onion is subject to significant stress and yield reduction caused by sap feeding onion thrips. In Kenya, the general control recommendation is to spray the crop with insecticides as soon as the pest appears and to continue thereafter, throughout the crop season. The practice is expensive and environmentally hazardous. Two field trials were conducted at the Kenya Agricultural Research Institute (KARI) Mwea-Tebere farm to identify the growth stages most susceptible to onion thrips infestation that would provide the highest economic benefits to the onion grower by managing thrips, with the aim of promoting need-based onion pesticide application. The trial design was randomized complete blocks of 16 treatments replicated three times. The treatments constituted insecticide treatments at different onion growth stages and frequencies. Thrips density/plant was estimated weekly through a destructive cutting and bagging method. Yield gains due to the application of the protection regimes were calculated and the marginal returns estimated as the income of yield gain divided by the cost of the control option. Thrips infestation was significantly (p=0.05) higher in the unprotected plots than all the protected plots in the first and second trial and resulted in significant yield losses of 59 and 29%, respectively. A significant onion yield response to thrips infestation was observed during the 2nd month (bulb-formation) and 3rd month (bulb-enlargement) after transplanting, but no significant response was observed in the 1st month (pre-bulbing) and 4th month (maturation) respectively. Onion protection only during the bulb-formation stage resulted in higher marginal returns than full season pesticide application. The studies suggested that control recommendations for onion thrips could be refined to optimize selective pesticide use in Kenya.
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