School of Pure and Applied Sciences
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Item Plasmid mediated penicillin and tetracycline resistance among Neisseria gonorrhoeae isolates from Kenya(2020) Kivata, Mary Wandia; Mbuchi, Margaret; Eyase, Fredrick; Bulimo, Wallace Dimbuson; Kyanya, Cecilia Katunge; Oundo, Valerie; Mbinda, Wilton Mwema; Sang, Willy; Andagalu, Ben; Soge, Olusegun O.; McClelland, Raymond Scott; Distelhorst, JohnBackground: Treatment of gonorrhea is complicated by the development of antimicrobial resistance in Neisseria gonorrhoeae (GC) to the antibiotics recommended for treatment. Knowledge on types of plasmids and the antibiotic resistance genes they harbor is useful in monitoring the emergence and spread of bacterial antibiotic resistance. In Kenya, studies on gonococcal antimicrobial resistance are few and data on plasmid mediated drug resistance is limited. The present study characterizes plasmid mediated resistance in N. gonorrhoeae isolates recovered from Kenya between 2013 and 2018. Methods: DNA was extracted from 36 sub-cultured GC isolates exhibiting varying drug resistance profiles. Whole genome sequencing was done on Illumina MiSeq platform and reads assembled de-novo using CLC Genomics Workbench. Genome annotation was performed using Rapid Annotation Subsystem Technology. Comparisons in identified antimicrobial resistance determinants were done using Bioedit sequence alignment editor. Results: Twenty-four (66.7%) isolates had both β-lactamase (TEM) and TetM encoding plasmids. 8.3% of the isolates lacked both TEM and TetM plasmids and had intermediate to susceptible penicillin and tetracycline MICs. Twenty-six (72%) isolates harbored TEM encoding plasmids. 25 of the TEM plasmids were of African type while one was an Asian type. Of the 36 isolates, 31 (86.1%) had TetM encoding plasmids, 30 of which harbored American TetM, whereas 1 carried a Dutch TetM. All analyzed isolates had non-mosaic penA alleles. All the isolates expressing TetM were tetracycline resistant (MIC> 1 mg/L) and had increased doxycycline MICs (up to 96 mg/L). All the isolates had S10 ribosomal protein V57M amino acid substitution associated with tetracycline resistance. No relation was observed between PenB and MtrR alterations and penicillin and tetracycline MICs.Item ICT for Sustainable Development without Digital Divide in Africa(2020-04-25)One of the most noteworthy challenges in society in the 21st century is sustainable development. The usefulness of ICT has led to an overwhelming demand for electronics such as computers and mobile phones. ICT is vital for poverty reduction and in the growth of sectors such as agriculture, business, health, education etc. Applying ICT to drive any development has its advantages and disadvantages. Therefore, its measurement should be focused on whether the benefits outweigh the pitfalls. The purpose of carrying out this research is because most development initiatives in Africa are being undertaken with the use of ICT. Therefore, this paper surveys the literature on ICT for sustainable development without the digital divide and how ICT can lead to un-sustainability in Africa. Findings indicate that driving sustainable development through ICT initiatives bring much greater benefits than negative consequence.Item Simplifying the Opaqueness and Dilemma of the Computing Disciplines for the Common Person in Society(2023-12) Mohammed Swaleh Mohammed; Wabwoba, FranklinThe computing fields have grown increasingly influential in modern times, yet their complexities and technical terms can prove problematic for the public to comprehend. This paper proposes to bridge this gap, introducing readers to the five major computing disciplines as per the Association of Computing Machinery (ACM), 2016: computer science, Information System, software engineering, computer engineering, and information technology. An additional more recent discipline which does not fall under the 5 ACM disciplines, Information Communication Technology was also introduced. The paper first examines the evolution of the Computing disciplines from the 1960s. Then the paper briefly discusses each discipline and how the disciplines overlap with each other through using secondary data materials. A section on the new and emerging computing technologies outlines the recognition status by the society. The interdisciplinary nature of the computing disciplines necessitated the need to simplify it. Thus, a table was done which summarized various works of various researchers who had researched on the computing disciplines.Item The Role of ICT in Social Inclusion: A Review of Literature(2023-01) Muchiri, Njoki; Wabwoba, FranklinThe questions we seek to answer in this paper are; Have ICT contributed to enhance social inclusion? Has use of ICT brought new forms of exclusion? And what is the way forward in using ICT for an inclusive society? Desk Top research was used. From the findings, one form of exclusion often leads to another, resulting to a cycle. a spanner and Nut Model was developed to show how Knowing which exclusion to reduce in order to break the cycle is critical in determining the type of ICT and how to apply it in order create an inclusive society.Item Usability Evaluation of Kenyan Universities Websites for Webometrics Ranking(2022-02) Mukanda, Adeline; Buguah, Samuel M.; Wabwoba, FranklinThe study aimed to evaluate the usability of Kenyan universities websites for webometrics ranking using web analysis tools. The target population for the study was the top five (5) universities in the January 2020 edition of webometrics ranking in Kenya. The study collected, analyzed, and evaluated website usability of the following website attributes: URL structure, Website Age, Uptime, Content Management System, website hosting service provider, Responsiveness, Browser compatibility, Color contrast, Domain Authority, Domain Backlinks, Website traffic, Broken Links, Number of Subdomains, Website Speed, Indexed webpages, Website compression, Search Engine Optimization, Website content utility, Website design and Website Accessibility. After analysis, it was noted that Kenyan universities need to improve on some of the usability criteria used in the study where a score of less than 50% was attained, such as Website compression, website speeds, website subdomain, website indexed pages, website traffic, website backlinks, website colour contrast and website design.Item On a Quotient Group 7^4:(3X2S_7) of a 7-local Subgroup of the Monster M(Karatina University, 2023-01) Musyoka, David Mwanzia; Njuguna, Lydia Nyambura; Prins, Abraham Love; Chikamai, LucyItem Faculty and students’ perspectives of online learning during COVID-19 crisis: Constraints and opportunities for Kenyan universities.(2023-04-15) Wekullo, Caroline S.; Kabindio, Brendah; Juma, IddiAt the onset of COVID-19 pandemic, all higher education institutions had to abruptly migrate from face-to-face mode of learning to virtual learning. Students and faculty members were thrown into the process of acquainting themselves with a fully virtual system. How prepared were institutions, faculty members, and students for the abrupt and massive transition to online learning? The study examined the faculty and students’ perspective of online learning during COVID-19 pandemic. Specifically, the study examined the perceptions of faculty and students on the preparedness of higher education institutions for online teaching and learning. The study also determined the perceptions of staff and students towards online education, constraints, and opportunities. A descriptive cross-sectional survey was conducted at a public university in Kenya. A semi-structured interview was conducted on a total sample of 36 respondents; 24 students and 12 faculty members to capture unique, idiosyncratic and whole individual viewpoints. A conventional Content Analysis was done to determine words or concepts within the transcripts. The findings showed that the study institution, faculty members, and students were unprepared for the immediate and massive shift to online teaching and learning and had challenges using virtual technologies for teaching and learning during the lockdown. These challenges ranged from insufficient or lack of internet bandwidth, training, increased teaching and learning workloads to limited time for interactions. Based on the findings, the study suggests significant implications for higher education institution leaders, policymakers, faculty, and students. The study contributes to the existing literature by analyzing the perceptions of faculty and students about the preparedness of institutions, faculty, and students on the abrupt shift to online learning during the COVID-19 pandemic.Item Mechanisms Used by Academic Libraries in Kenya to Enhance Webometrics Ranking(2023-04) wamahiga, George M.Libraries essentially provide equal and fair access to information. Thus, they have a significant influence on the Webometrics ranking of universities across the world. Academic libraries affect Webometrics ranking through the generation of scientific publications and other scholarly resources. The purpose of this study was to examine mechanisms used by academic libraries in Kenya to enhance Webometrics ranking and to analyse the extent to which Webometrics ranking mechanisms in place in university libraries in Kenya have been successful. This study adopted a mixed research design. The target population included staff members of the University of Nairobi, Chuka University, and Kirinyaga University. A total of 73 staff members from the library, ICT, and research departments involved in Webometrics ranking were selected purposively. Semi-structured questionnaires were used as the data collection instruments. Quantitative data was analysed descriptively while qualitative data were analysed using content analysis. The study established that mechanisms put in place to enhance the participation of university libraries in Kenya in the Webometrics ranking include improved use and submission of e-resources as well as promotion of high-quality research in the institutions. Webometrics ranking mechanisms currently in place at the university libraries in Kenya have only been moderately successful. The findings of this study may be used by other institutions of higher learning on the mechanisms they can adopt to increase the capacities of their academic libraries so as to improve the contribution of these libraries to Webometrics and consequently to the overall ranking of the individual universities nationally and internationally.Item An Enhanced Data Transmission in Mobile Banking Using LSB-AES Algorithm.(2023-06-03) Okari, Daniel O.; Awuor, Fredrick Mzee; Makiya, Ratemo; Oduor, CollinsItem Green synthesis of copper oxide nanoparticles and its efficiency in degradation of rifampicin antibiotic.(Springer, 2023-08-28) Nzilu, Dennis Mwanza; Madivoli, Edwin Shigwenya; Makhanu, David Sujee; Wanakai, Sammy Indire; Kiprono, Gideon Kirui; Kareru, Patrick GachokiIn recent ages, green nanotechnology has gained attraction in the synthesis of metallic nanoparticles due to their cost-effectiveness, simple preparation steps, and environmentally-friendly. In the present study, copper oxide nanoparticles (CuO NPs) were prepared using Parthenium hysterophorus whole plant aqueous extract as a reducing, stabilizing, and capping agent. The CuO NPs were characterized via UV–Vis Spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), powder X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Dynamic Light Scattering (DLS). The UV–Vis spectra of CuO NPs showed a surface plasmonic resonance band to occur at 340 nm. FTIR analysis revealed the presence of secondary metabolites on the surface of CuO NPs, with a characteristic Cu–O stretching band being identified at 522 cm−1. Scanning electron micrographs and transmission electron micrographs showed that CuO NPs were nearly spherical, with an average particle of 59.99 nm obtained from the SEM micrograph. The monoclinic crystalline structure of CuO NPs was confirmed using XRD, and crystallite size calculated using the Scherrer-Debye equation was found to be 31.58 nm. DLS showed the presence of nanoparticle agglomeration, which revealed uniformity of the CuO NPs. Furthermore, the degradation ability of biosynthesized nanoparticles was investigated against rifampicin antibiotic. The results showed that the optimum degradation efficiency of rifampicin at 98.43% was obtained at 65℃ temperature, 50 mg dosage of CuO NPs, 10 mg/L concentration of rifampicin solution, and rifampicin solution at pH 2 in 8 min. From this study, it can be concluded that CuO NPs synthesized from Parthenium hysterophorus aqueous extract are promising in the remediation of environmental pollution from antibiotics. In this light, the study reports that Parthenium hysterophorus-mediated green synthesis of CuO NPs can effectively address environmental pollution in cost-effective, eco-friendly, and sustainable ways.
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