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Item Evaluation of Ephestia kuehniella and Corcyra cephalonica as hosts for mass rearing trichogramma species nr. Mwanzai and trichogrammatoidea species nr. Lutea(2008-01-16) Migiro, L. N.; Gitonga, L. M.; Sithanantham, S.Production of vegetables free of chemical pesticides requires, among other things, use of biocontrol agents that can be effectively and efficiently produced in large quantities. Two factitious hosts were evaluated for their suitability in mass rearing of two native Trichogrammatids. Egg cards bearing host eggs were separately offered to female parasitoids in glass vials and allowed to parasitise for 24 hours. New egg cards were offered daily till death of the female parasitoid. The number of eggs parasitised, emerged adult progeny and female progeny differed significantly between the two parasitoids, with Trichogramma sp. nr. Mwanzai appearing to be superior to Trichogrammatoidea sp. nr. lutea.There were no differences in the mass production efficiency and quality of progeny between the Ephestia kuehniella and Corcyra cephalonica. The two hosts can be effectively used for mass production of either of the parasitoids.Item Biological control agents of aphids (Homoptera: Aphididae) on potatoes (Solanum tuberosumL) in Kenya(2010) Machangi, J. M.; Gitonga, L. M.; Nderitu, J. H.; Maniania, N. K.; Kabira, J. N.A field survey was conducted in four major potato growing areas in Kenya (Kiambu, Nyandarua, Meru and Molo Districts) during January and February, 2008 (short rains crop) and June 2008 (long rains crop) to determine the occurrence of predators, parasitoids and pathogens of the aphids Myzus persicae Sulzer and Aphis gossypii Glover in potato crops. In each of the four areas, 30 potato farms distributed in different parts of the survey area were selected at random for the surveys. Insects collected were brought to the laboratories at the International Centre of insect physiology and Ecology (icipe), Nairobi, for identification and fungal infection. For isolation of fungal pathogens, dead aphids were transferred on moist filter paper placed on sterile Petri dishes to allow the growth of the fungus on the surface of the cadaver, after which fungus was transferred on artificial media for isolation. Four aphid species, M. persicae, A. gossypii, Macrosiphum euphorbiae Thomas and Aulacorthum solani Kaltenbach were identified in all the four survey areas. Sixteen predator species of the aphids were identified. The most prevalent were the ladybeetles Harmonia axyridis (Pallas) and Hippodamnia convergens Guérin-Méneville (Coleoptera: Coccinellidae), followed by the minute pirate bugs, Orius spp. (Heteroptera: Anthocoridae) and the aphid eating gall midge Aphidoletes aphidimyza Rondani (Diptera: Cecidomyiidae). Three hymenopteran parasitoid species (Braconids followed by Chalcids then the Ichneumonids) and four fungal pathogen species, Beauveria bassiana Balsamo (Ascomycota: Hypocreales), followed by Verticillium lecanii Zimmermann (Hypocreales: Incertae sedis), Metarhizium anisopliae (Metsch.) Sorokin (Deuteromycotina: Hyphomycetes) then Pandora neoaphidis (Remaudière &Hennebert) Humber (Zygomycetes:Entomophthorales) were identified in all four areas of the field survey.Item Evaluation of feed for rearing corcyracephalonica and optimisation of host and parasitoid ages for mass-production of trichogramatid parasitoids(2008) Migiro, L. N.; Gitonga, L. M.; Sithanantham, S.Item The Critical Onion Growth Stages For Selective Protection From Thrips Tabaci Lindeman (Thysanoptera:Thripidae) Infestation(Kenya Agricultural Research Institute, Nairobi (Kenya), 2006-01) Waiganjo, M. M.; Sithanantham, S.; Mueke, J. M.; Gitonga, L. M.Field trials were conducted during 2000-2001 at KARIandrsquo;s Mwea-Tebere and Kiboko farms to establish the effect of onion thrips, Thrips tabaci Lindeman on onion bulb yields and to identify the critical onion growth stage for selective protection from the pest infestation. Yield reductions of 59 and 27% were recorded in the first and second crops respectively at Mwea-Tebere, but no significant yield reductions were observed in a third crop grown at Kiboko with low thrips infestation. A significant onion yield response to thrips infestation was observed during the 2nd month after transplanting (bulb-formation) and 3rd month after transplanting (bulb-enlargement), but no significant response was observed during the 1st month (pre-bulbing) and 4th month (maturation stage). Onion protection during bulb-formation and enlargement stages only, resulted in higher marginal returns than full season pesticide application. The studies suggest that control recommendations for onion could be refined to optimize need-based protection.Item Fishing power of conventionally harvested wetland baitworms compared to black soldier fly larvae as alternative baits in tropical artisanal hook fishery(2018) Kihia, C. M.; Gitonga, L. M.; Tembo, J. E.; Kanyeki, E.; Munguti, J.; Muli, B.Extensive and intensive, harvesting of earthworms and polychaetes from wetland substrate, for artisanal hook fishery, affect structural and functional integrity of these critical habitats. Cultured Black Soldier Fly larvae (BSF, Hermitia illucens), are suggested as sustainable alternatives. This study compares bait and landed fish properties of wild earthworm (Eisinia sp) and polychaete (Marphysa mossambica), with cultured BSF larvae as bait, in hook fishery. Participating fishers, were supplied with known quantity of bait, and the duration and quantity of fish obtained, monitored. Hook casting, attractiveness and killing power, as well as fish quality and quantity, were computed and compared among sites and bait. Results reveal significant (P<0.05) differences in bait and landed fish properties. Lower bait casting (8.8%), but higher attractiveness (0.96) and killing power (55%), were obtained using polychaete in marine habitats, than either earthworms or BSF. Similarly, higher fish landings s (0.59 kg.hr-1), were obtained using polychaete, than comparable BSF or earthworm (<0.1 kg. hr-1. We surmise that wild baitworms, are easier to handle, attract and land more fish, leading to higher variety and abundant landed fish, than cultured BSF larvae. Lower performance of BSF is attributed to; poor response of target fish to imprinted physical and chemical attributes of the bait. This may imply that fishers, have limited justification for substituting cultured BSF, with conventional wild baitworm in artisanal fishery. Further elucidation of drivers to bait choice and performance coupled with improvement in cultured bait quality might, provide sustainable solutions.Item Effect of temperature on the development of Orius albidipennis Reuter, a predator of the African legume flower thrips, Megalurothrips sjostedti Trybom(2002) Gitonga, L. M.; Löhr, B.; Overholt, W. A.; Magambo, J. K.; Mueke, J. M.The effects of temperature on the development of Orius albidipennis (Reuter) (Hemiptera: Anthocoridae), reared on its prey, Megalurothrips sjostedti (Trybom) (Thysanoptera: Thripidae), were studied in the laboratory. Nymphal mortality was 87, 48 and 38 % at 20, 25 and 30 °C, respectively. With the exception of first-instar nymphs, percentage mortality was lowest at 25 °C. Pre-oviposition period and longevity decreased with an increase in temperature. Mean daily and total fecundity were 2.1 and 76.4 respectively at 25 °C. The mean developmental period from oviposition to adult eclosion was 27.7, 14.1 and 10.9 days at 20, 25 and 30 °C respectively. There was a linear relationship between temperature and developmental rate (1 /day) of O. albidipennis. Lower thermal thresholds were 13.8, 13.5, 12.7, 15.0, 13.8 and 12.5 °C for eggs and nymphal stages 1 to 5, respectively, with the corresponding average degree-day requirement of 46.9, 42.6, 33.3, 29.2, 26.1 and 51.4 days, respectively. The implications of these results for biological control of thrips are discussed.Item Open relationships in the castles of clay: high diversity and low host specificity of Termitomyces fungi associated with fungus-growing termites in Africa(International Union for the Study of Social Insects (IUSSI), 2010-08) Lagat, Zipporah Osiemo; Marten, Andreas; Kaib, M.; Gitonga, L. M.; Boga, H. I.; Brandl, R.In the African and Asian tropics, termites of the subfamily Macrotermitinae play a major role in the decomposition of dead plant material. Their ecological success lies in the obligate mutualism of the termites with fungi of the genus Termitomyces. Before the advent of molecular studies, the interaction with these fungi was poorly understood. Here, we combined available ITS sequence data from West, Central, and South Africa with data of 39 new samples from East Africa to achieve the most comprehensive view of the diversity and host specificity of Termitomyces symbionts across Africa to date. A high amount of sequence divergence in the ITS sequences was found; 11 different Termitomyces lineages in East Africa and >30 lineages across Africa were identified, and the expected diversity is estimated to be about 41 lineages. The fungal lineages belong to four major clades, each almost exclusively associated with one termite host genus. Analysis of molecular variance revealed that 40% of the ITS sequence variation occurred between host genera, indicating close co-evolution at this level. However, within host genera, fungal lineages and haplotypes were frequently shared among host species and sampling localities, except for fungal symbionts of Odontotermes. Horizontal transmission of fungal symbionts may facilitate the transfer of haplotypes and species among hosts. However, at present, we have little understanding of the maintenance of specificity at the genus level. Possible explanations range from substrate specificity of fungi to an active selection of fungi by termites.Item Host plant resistance among tomato accessions to the spider mite Tetranychusevansi in Kenya(Springer, 2011-04-11) Onyambus, G. K.; Maranga, R. O.; Gitonga, L. M.; Knapp, M.The spider mite Tetranychus evansi has a broad range of host plants. Control of T. evansi has been a big challenge to tomato farmers due to its fast rate of reproduction, development of resistance to chemical pesticides and its ability to use weeds as alternative hosts when the tomato plants are not available. The aim of the current study was to determine the host plant acceptance and the relative contributions of trichomes in the control of the red spider mite by comparing the survival, development and oviposition rates of the red spider mite on eight tomato accessions. Leaflets from eight tomato varieties were assayed with the spider mites to determine the egg laying capacity and developmental time of the spider mites on the tomato accessions as well as the trichome densities. Densities of trichome types I, IV, V and VI varied among the tomato accessions. Variation in types I, IV and VI accounted for most of the variation in mite responses. The varieties with high densities of types IV and VI had the highest fecundity and mite development did not go beyond the larval stage. The developmental time varied significantly among the tomato accessions. The results indicated that the higher the density of trichome type I the lower the adult survival. The findings indicated possible resistance of some of the tested tomato accessions against T. evansi which is partially associated with trichomes types and density.Item Performance of a semiochemical-baited autoinoculation device treated with Metarhizium anisopliae for control of Frankliniella occidentalis on French bean in field cages(Karatina University, 2011-12-16) Niassy, S.; Maniania, N. K.; Subramanian, S.; Gitonga, L. M.; Ekesi, S.The performance of an autoinoculation device was evaluated in field cage experiments for control of Western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), in French bean, Phaseolus vulgaris L. var. Samantha (Fabaceae). Treatments consisted of a fungus-treated device with and without semiochemical (Lurem-TR), and a fungusfree device as control; the fungus used was Metarhizium anisopliae (Metchnikoff) Sorokin (Hypocreales: Clavicipitaceae). The overall mean number of conidia acquired by a single insect was higher in the fungus-treated semiochemical-baited device than in the device without 10 × 0.6 ± semiochemical: 5.04 10 × 0.4 ± vs. 2.24 conidia per insect. The overall thrips mortality was also higher in the semiochemical-baited device than in the device without the 3.5%. Conidial viability was not ± 3.9 vs. 41.7 ± semiochemical: 59.3 affected in the M. anisopliae-treated device without days after treatment, whereas it was considerably semiochemicals, 7 days post-inoculation 1.1%, 2 and 7 ± 1.3 to 6.5 ± decreased – from 81.0 – in the M. anisopliae-treated semiochemical-baited device. Thrips mortality was positively correlated with M. anisopliae conidial viability, and conidial viability was negatively correlated with conidial acquisition. This suggests that the semiochemical volatiles reduced the conidial viability, which in turn resulted in significant reduction in thrips mortality, despite the highest conidial acquisition in the semiochemical-baited fungus-treated device. Thrips density per plant was significantly reduced in both fungus-treated 1.7 adults per plant; without ± treatments (with semiochemical: 8.7 1.4 adults per plant) compared with the fungus-free ± semiochemical: 6.6 2.6 adults per plant). These results demonstrate the ± control (19.8 potential for an autoinoculation device strategy for the control of thrips, particularly in screenhouses.